Deleted
Sample Discontinued
Experience: None
Not Used
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Part:BBa_K1678000:Design

Designed by: Antoine Vigouroux   Group: iGEM15_Paris_Bettencourt   (2015-09-08)


Promoter from BBa_J23119 followed by 4 orthogonal LoxP sites


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 2029
    Illegal SpeI site found at 2281
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2029
    Illegal NheI site found at 2057
    Illegal NheI site found at 2080
    Illegal SpeI site found at 2281
    Illegal NotI site found at 2035
    Illegal NotI site found at 2288
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2029
    Illegal XhoI site found at 1013
    Illegal XhoI site found at 1905
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 2029
    Illegal SpeI site found at 2281
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 2029
    Illegal XbaI site found at 2044
    Illegal SpeI site found at 2281
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Fillers are present in between the LoxP sites to make it easier to synthesize and allow for PCR primers design.


Source

Gene synthesis.

References